Requirements relative to Sequencing projects

General notes

To guarantee the normal processing of your project, samples should meet the standards given below. If your samples do not meet these standards, and you are unable to produce higher-quality samples, please contact us before shipping your samples.

Sample Requirements


Library typeSample TypeRecommended amountAmount required VolumeConcentrationRNA integrity number (Agilent 2100)Purity
Eukaryotic RNA-Seq Total RNA (Animal) ≥ 2 μg ≥ 1 μg ≥ 20 μL ≥ 50 ng/μL ≥ 6.8, smooth base line No degradation, no contamination
Total RNA (Plant and Fungus) ≥ 2 μg ≥ 1 μg ≥ 20 μL ≥ 50 ng/μL ≥ 6.3, smooth base line
Prokaryotic RNA-Seq Total RNA ≥ 6 μg ≥ 3 μg ≥ 20 μL ≥ 50 ng/μL ≥ 6.0, smooth base line
Eukaryotic Long non-coding RNA Sequencing Total RNA (Animal) ≥ 4 μg ≥ 2 μg ≥ 20 μL ≥ 50 ng/μL ≥ 6.8, smooth base line
Total RNA (Plant and Fungus) ≥ 4 μg ≥ 2 μg ≥ 20 μL ≥ 50 ng/μL ≥ 6.3, smooth base line
Eukaryotic small RNA Sequencing Total RNA (Animal) ≥ 6 μg ≥ 3 μg ≥ 20 μL ≥ 50 ng/μL ≥ 8, smooth base line
Total RNA (Plant and Fungus) ≥ 6 μg ≥ 3 μg ≥ 20 μL ≥ 50 ng/μL ≥ 7.5, smooth base line


Library typeSample TypeRecommended amountAmount required VolumeConcentrationPurity
Human Whole Genome/Exome Sequencing Genomic DNA ≥ 2 μg ≥ 1 μg ≥ 20 μL ≥ 20 ng/μL No degradation, no contamination
Genomic DNA (PCR-Free) ≥ 3 μg ≥ 1.5 μg ≥ 20 μL ≥ 20 ng/μL
Whole Genome Bisulfite Sequencing Genomic DNA ≥ 6 μg ≥ 3 μg ≥ 20 μL ≥ 50 ng/μL
ChIP-Seq ChIP-Seq DNA ≥ 100 ng ≥ 50 ng ≥ 10 μL ≥ 20 ng/μL Main peak of 100 bp – 500 bp

Pre-Quality Control (QC) Instructions

Customers must provide the results of analysis of sample quality obtained using one of the following methods: Qubit®, NanoDrop™, agarose gel electrophoresis, or Agilent 2100. It is recommended samples be analyzed by Qubit/PicoGreen/gel electrophoresis (with quantity indicator), NanoDrop™ quantification is discouraged. If NanoDrop™ is utilized for pre-QC quantification, we strongly recommends that you send more DNA/RNA for processing than the amounts given above.

For gel electrophoresis, the following conditions are recommended:

Note: Different electrophoresis conditions may generate a different, and potentially misleading, QC report on your samples. Therefore, it is highly recommended that you adhere to the conditions recommended above for the initial check, and that you provide a picture of the gel.

Sample Labeling Recommendations

Sample Packing Recommendations

Shipping Recommendations

Disclaimer: The information below only constitutes a recommendation for shipping samples classified as “non-regulated materials”. Due to continuing changes in regulations, customers should always check with their safety office and/or shipping department to ensure regulatory compliance.


  1. It is highly recommended that RNA samples be shipped in dry ice packaging. Other packaging/transportation methods may add impurities or cause slight degradation of the RNA.

  2. The quantity of dry ice and ice bags needed varies with the season (i.e., room temperature), transit time, and the thickness of Styrofoam box and receptacle. Please contact your local courier office for estimated transit time. Normally, dry ice is consumed (sublimates) at a rate of 5 kg (11 pounds) per day.

Qubit is a trademark of Life Technologies and Thermo Fisher Scientific.
NanoDrop is a trademark of NanoDrop Technologies LLC.
Agilent 2100 Bioanalyzer is a trademark of Agilent Technologies.